Category Archives: Anti-aging

Bio-Skinflora Solution

Description: nutrient solution with strong capacities of moisture retention, soothing allergies and anti-irritation

Innovative Mechanism:

Plant collagen, glucan and prebiotics work together to form a highly hydrophilic micro-ecological protective membrane on the skin, which protects, repairs and strengthens the first defensive line for skin.

With a strong hydrophilic feature, phytocollagen is a very good natural moisturizing ingredient. Unlike general covering moisturizer, Plant collagen can promote the keratinocytes of skin to produce a natural moisturizing factor (NMF), which exerts a moisturizing effect, penetrates and infiltrates the stratum corneum, making the skin more transparent and moisturized, and forming a skin self-protection mechanism. This will enhance skin elasticity, eliminate small wrinkles, improves the texture of skin, and isolates external toxic and harmful substances.

The natural water-soluble yeast β-glucan can bind to the receptor CR3 of yeast β-glucan on the Langerhans cells in the epidermis, activate macrophages, and produce various cytokines (e.g., IL-1, IL-6, GM-CSF), epidermal growth factor (EGF), tumor necrosis factor (TNF-α) and angiogenic factors (AF), etc., thus improving skinimmunity. Yeast glucan also has the function of anti-inflammation, anti-allergy, eliminating free radical, moisturizing and accelerating stratum corneum renewal, which helps the skin resist external mechanical and chemical stimuli and facilitates the recovery of injuries. An increase in epidermal growth factor can promote the production of collagen and elastin, thus improving the exterior of skin and eliminating fine line wrinkles.

Prebiotics promotes the growth of epidermal probiotics through selective nutrient supply mechanisms, inhibits the growth of harmful flora through competitive mechanisms, establishes and maintains a stable and healthy skin micro-ecological system, maintains healthy skin pH, and reduces inflammation and allergic reactions. .

 

 

 

 

 

 

 

Fermentation-derived Phytocollagen

 

 

 

 

 

 

 

 

 

Plant-derived Fructan and Fructose Oligomers

 

 

 

 

 

 

 

Yeast-derived Glucan

 

Prebiotics can improve skin micro-ecological balance

Applications:

  1. Sensitive skin: protecting the skin from external stimuli, soothing allergies and anti-inflammation
  2. Dry skin: moisturizing, wrinkle-reducing, anti-aging, improving skin texture
  3. Anti-acne&pimple: inhibiting the growth of harmful microbes such as propionibacterium acnes, promoting the growth of skin probiotics, establishing a healthy micro-ecological system, and regulating the pH value of skin health.
  4. Infant care: prevention on infection (especially for the areas wrapped with diaper)
  5. Other skincare and haircare products.

 

Instruction for Prescription

Solubility: This product is easily soluble in water

Recommended dosage: The product is added in an amount of 1-10%. It is recommended to add this below 60℃ in the final stage of cosmetic production. It is compatible with a wide range of cosmetic ingredients.

 

 

PuriBifido

INCI Name :Bifida Ferment Lysate

Bifida Ferment Extract, Phenoxyethanol, Potassium Sorbate, Xanthan Gum

CAS No. None for now; 122-99-6; 24634-61-5; 11138-66-2

It is fermented by the compound bifidobacteria (known as bifidus yeast in the industry). It is a probiotic product that thoroughly improves the skin condition and can be classified as a skin micro-ecological active matter. The core ingredient of the Estee Lauder Advanced Night Repair is the bifidus yeast. The anti-ageing active ingredients extracted from the lysate of bifidobacteria through biotechnology can effectively protect skin, reduce the damage caused by ultraviolet rays to the skin, activate the immuno-suppressive process, stimulate endogenous skin protection, and promote cell repair. It can reduce skin spots, brighten the skin, reduce the number and degree of wrinkles, and smooth the skin. Bifidus yeast can promote cell repair and support the endogenous protection mechanism to fight against skin aging.

PuriBifido is created through the fermentation of composite bifidobacterium (commonly known as Bifida) groups. It is a bifidobacterium derivative that can completely improve skin states with the

Specification

Exterior
Opaque light yellow fluid
Smell
Slight featured acid smell
pH
4.0~6.0
Total solids
≥ 3.0 %
Nitrogen level
0.1~0.15%
Total amount of fungi
≤ 50 CFU/ml
Total amount of bacteria
≤ 300 CFU/ml
Heavy metal
≤ 40 ppm

Recommendation for use

Recommend dosage: 5.0~20.0%. An addition may be applied if required.

  • This product is a suspension, so there may be a few sediments after being left still for a long time, which is normal. Please shake it up before use.
  • It is recommended to add thickener in the prescription so as to prevent bacteria pieces from subsiding. The specific prescription shall be determined after testing.
  • Use it at the temperature lower than 60℃, and add it to the aqueous phase.
  • If this product is mixed with high-concentration alcohols, the flocculation precipitation will occur. The maximum allowable alcohol concentration is 30% according to the prescription.
  • If this product contacts a strongly acidic/alkaline environment, the flocculation precipitation and color change will occur. It should be avoided to directly add the product to such environment.
  • Nano Liposomal Vitamin A (NanoActive A)

    NanoActive A is a novel vitamin A nanoliposome preparation (NanoLiposomal A).
    Product Name: Nano Liposomal Vitamin A: Nanoactive Vitamin A; BioaActive Vitamin A; BioActive VA; NanoActive VA; Soluble Vitamin A CAS: 79-81-2

    Description: Vitamin A palmitate is a vitamin A derivative and a fat-soluble antioxidant. NanoActive A is water-soluble, transparent,highly effective and non-irritating vitamin A, which can repair cells and protect against UV-induced skin aging, promote the metabolism of epidermis and keratinocytes, facilitate and strengthen the exfoliation of keratinocytes and the growth of new cells, as well as the production of collagen and elastic fibers, while helping to maintain the normal metabolic cycle of cells.

    Chemical structure of Vitamin A

    Mechanism

     Features

  • NanoActive A uses FDA GRAS grade materials for high safety
  • NanoActive A is stable to heat, pH and shearing, so it is convenient to use.
  • NanoActive A is 100% water soluble and 100% oil soluble (amphiphilic), allowing a wide range of uses.
  • NanoActive A greatly improves the stability and bioavailability of vitamin A
  •  

    Efficacy:

  • NanoActive A has the ability to regulate the metabolism of the epidermis and stratum corneum, which can resist aging and wrinkle
  • NanoActive A relieves seborrhea and makes skin elastic, while diminishing spots and softening skin
  • NanoActive A helps protect the epidermis and mucous membranes from bacteria in order to maintain skin health
  • NanoActive A can improve the stability of other active matters in the product and facilitate absorption
  • Effectively increase skin elasticity

     

    Use:Add it at room temperature. You may heat it to over 40℃ to speed up the dissolution. Recommended dosage: 1-5%. Metal ions such as iron ions must be avoided in the system. Better effect with products such as vitamin C and vitamin E.

    Storage: Keep it away from light at a temperature below 25℃.

    NanoActive RAL

    Nanoactive RAL, INCI name: Retinal, CAS: 116-31-4.

    Product Name: Nanoactive RAL, BioaActive Retinaldehyde; BioActive Retinal; Soluble Retinal; Soluble Retinaldehyde;Nano Liposomal

    Nanoactive RAL is a novel retinal nanoliposome formulation (NanoLiposomal RAL).

    A new liposome preparation. RAL has the characteristics of retinoic acid in the skin, and is better tolerated than retinoic acid. It directly interacts with protein receptors in cells, stimulates epidermis to accelerate regeneration, increases the thickness of the stratum corneum, and makes the skin tighter and more orderly. It also accelerates the production of collagen in the dermis layer, prevents the damage of UV to collagen, inhibits the increase of MMP caused by UV, and makes the skin more elastic; the unique aldehyde-based structure has a very good effect on P. acnes, etc., and effectively improves acne skin. Large amounts of retinal, retinol, retinol retinate (15498-86-9), HPR(893412-73-2) and other Vitamin A kind products of pharmaceutical grade are available.

     

    Description:

    Retinaldehyde (RAL), also known as vitamin A aldehyde, is a derivative of retinol after oxidation. It is formed by oxidative cleavage of β-carotene. If  reduced, retinol can be attained; If oxidized, retinoic acid can be attained.Retinoic acid。”. The role of retinoic acid (Vitamin A) in skin diseaseis very extensive, but due to local irritation, its clinical application is limited to some extent.Retinaldehyde is an intermediate metabolite of natural retinoic acid and has similar biological activity as retinoic acid. But the skin is significantly more tolerant to retinoic acid.

    The main forms of vitamin A and their interconversion

    Retinal has many target on the skin. It can be used as a skin care product to produce a variety of effects such as anti-aging, skin rejuvenation, whitening, anti-wrinkle, acne, keratin, etc. It is an emerging high-efficiency natural active substance. Retinal is hardly soluble in water while easy to be oxidized and discolored, which limits its application in cosmetics. Nano actives with a particle size of less than 30 nm developed by nano-transmission system (NDS) enable retinals to be steadily wrapped in nano-carriers, greatly improving the absorption efficiency and bioavailability. This overcomes the original defects and maintain the advantages and enables a more convenient and wider range of uses.

    Skin care effect

    The large use of non-physiological doses of exogenous retinoic acid can cause an overload of the retinoic acid-dependent pathway in the skin, causing adverse reactions such as skin irritation. RAL is a natural metabolite of retinol and a direct precursor of retinoic acid, and it also has similar biological activity to retinoic acid. The advantage of RAL compared with retinoic acid is its multi-directional metabolism, that is, the excess RAL can be rapidly reduced to retinol, then stored and inactivated in the form of retinol, and this “reverse metabolism” is not present in retinoic acid. After it is absorbed by the skin, most of it is converted to retinol vinegar stored for slow release, and a small amount of RAL is oxidized to retinoic acid by keratinocytes in differentiation-dependent manner, while a very small amount of retinoic acid is can exert significant biological effects in cells. This can avoid skin adverse reactions caused by high doses of retinoic acid. Clinical observations also show that topical RAL creams are well tolerated compared with retinoic acid and can be applied to sensitive areas. The topical RAL cannot absorbed by human blood. The levels of various vitamin A acids in the blood also cannot be affected.

    RAL needs to be activated by the conversion of enzymes in keratinocytes to retinoic acid, so RAL has similar biological activity as retinoic acid. One of the indicators of retinoic acid activity is to induce the expression of retinoic acid binding protein (CRABP-2) in cells. External RAL can evidently induce the expression of CRABP-2 mRNA and protein. The topical natural retinoic acidcan also induce the expression of nuclear receptor in retinoic acid . Like retinoic acid, RAL also functions to regulate cell proliferation and differentiation. It can increase epidermal DNA synthesis, increase epidermis thickness, induce 50kD keratin, and reduce 7OkD keratin mRNA expression. Inducing positive keratinization, reducing 65kD keratin mRNA expression, and increasing filaggrin and loricin mRNA expression. In addition, RAL is also involved in the regulation of neovascularization in the skin, inhibiting the production of vascular endothelial growth factor by keratinocytes. It can repair the elastic fibers and collagen damage induced by UVA. RAL is also capable of dissolving acne and the direct sterilizing effect towards propionibacterium acnes. Natural retinoic acid play an important role in epidermal differentiation, dermal remodeling and curing skin inflammation. Retinaldehyde as an important intermediate metabolite of natural retinoic acid has both biological activity similar to that of retinoic acid and is well tolerated by the skin, thus it is given wider application in cosmetics.

    Retinaldehyde can repair elastic fiber and collagen loss caused by UV:

    Retinaldehyde prevents skin aging caused by ultrasound and fluid

    technology:

     

    Retinaldehyde can effectively improve skin photoaging symptoms:

     

     

    Retinaldehyde and retinal are well tolerated, while retinoic acid is more irritating

    Retinaldehyde can effectively inhibit epidermal melanin

    Features

  • NanoActive Retinaldehyde uses FDA GRAS grade materials for high safety
  • NanoActive Retinaldehyde has good heat, pH and sheer stability and is easy to use.
  • NanoActive Retinaldehydegreatly enhances the stability and bioavailability of retinal
  • NanoActive Retinaldehyde can improve the stability of other active matters in the product and facilitate absorption
  • NanoActive Retinaldehyde is 100% water soluble and 100% oil soluble, allowing a wide range of uses.
  •  

    Applications

  • Wrinkle repairing: promote collagen and hyaluronic acid synthesis, increase skin thickness and elasticity, repair the wrinkle and firm the skin.
  • Whitening and freckle removing: inhibit melanin synthesis, reduce pigmentation, whiten the skin and remove the freckle.
  • Anti-aging: anti-oxidation, anti-skin-aging
  • Skin-refreshing: renewal of keratin, refresh the skin.
  • Acne Treatment: sterilizing propionibacterium acnes and streptococcus, etc., treating acne and removing acne marks
  • Curing of skin photoaging
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     Use: Add it at room temperature; recommended dosage: 1-5% The optimum pH range of the system is 3.0-6.5, and the finished product should avoid direct exposure to sunlight (using opaque packaging). It is recommended to add UV absorber to day care products.

     

    Storage: keep it away from light at a temperature below 25℃.

     

    Nano-Lipoic Acid (NanoActive ALA)

    Nanoactive ALA is a new type of lipoic acid nanoliposome preparation (NanoLiposomal ALA).
    Product Name: Nano Liposomal ALA; Nanoactive ALA; BioaActive ALA; BioActive Alpha lipoic acid; NanoActive Alpha lipoic acid; Soluble alpha lipoic acid; Soluble ALA CAS: 62-46-4

    Description: Alpha lipoic acid is an enzyme existing in mitochondria, which is similar to vitamins and can  eliminate free radicals that accelerate aging and cause disease. Lipoic acid has both fat-soluble and water-soluble properties, so it can pass through the entire body and reach every cell, providing comprehensive performance for human body. It is an all-purpose antioxidant with fat solubility and water solubility. Lipoic acid has the antioxidant effects 400 times greater than that of vitamins C and E, and it can greatly enhance the effects of vitamins C and E. Also, its efficacy lasts longer than other antioxidants. Smaller than the molecules of vitamin E, it can be easily absorbed by the skin and be effective in beautifying, activating cells and facilitating hair growth. Lipoic acid is effective in removing dark eye circles, wrinkles and spots. In addition, it enhances metabolism, improving the body’s blood circulation, so that skill will no longer be dull and the appearance or pores will be minimized, making your skin enviously firm and delicate. Therefore, lipoic acid is also an anti-aging nutrient that ranks the first place in the United States, which ties with ALA. The lipoic acid nanoactive material with particle size less than 30nm developed by nano-transmission system (NDS) completely solves the problem of solubility, and allows lipoic acid to be stably encapsulated in nano-carriers, which can greatly increase its absorption efficiency and bioavailability.

    Lipoic acid’s structure

     

     

    Lipoic acid is an powerful versatile anti-oxidant that can regenerates other antioxidants.

     

    Lipoic acid (LA) target against skin aging

    Features

  • NanoActive ALA uses FDA GRAS grade materials for high safety.
  • NanoActive ALA has good heat, pH and shear stability and is easy to use
  • NanoActive ALA greatly improves ALA’s stability and bioavailability
  • NanoActive ALA improves the stability and absorption of other actives in the product.
  • NanoActive ALA is 100% water soluble and 100% oil soluble (amphiphilic) for a wide range of applications.
  •  

    Efficacy

  • NanoActive ALA can enhance the anti-oxidation effect of skin cells, reduce the damage from free radicals to the skin, significantly reduce skin roughness, eliminate freckles and reduce the appearance of fine lines.
  • NanoActive ALA is an active functional ingredient that is effective in anti-wrinkles, whitening, anti-aging, moisturizing and increasing skin elasticity.
  • It helps to enhance the efficacy of antioxidants such as polyphenols and kinetin, and also reduces the endogenous antioxidants.
  •    Use: Add it at room temperature. You may heat it to above 40 °C to speed up its dissolution, Recommended dosage: 1-3%. Better effect with products containing lipoic acid.

    Storage: Keep it away from light at a temperature below 25°C.

     

     

    Experimental proof

    Effect of lipoic acid on cell activity

    Lipoic acid’s whitening effect

    Lipoic acid’s anti-wrinkle effect

    NanoStemCellTM

         NanoStemCell anti-aging repair solution (NanoStemCellTM) is a novel skincare material developed by PuriPharm’s chief scientist Professor Robert J Lee and his team using plant extract, modern biomedicine and nano-transmission technology. It resists UV rays and blue laser radiation, and is featured with the functions of anti-oxidation, promoting extracellular stroma synthesis and repairing the stem cells of skin, thus acting as an anti-pollution and anti-aging repair from within. It is a new type of 4D anti-aging and wrinkle removing solution, applicable to deep anti-wrinkle, preventing dark eye circles, controlling oil, eliminating acnes, anti-aging and protection from blue lasers.

    Salvia miltiorrhiza and Buddleja officinalis, the traditional Chinese medicinal materials, contain a large number of functional compounds with good biological activity and significant pharmacological features. The PuriPharm team uses modern biotechnology to screen and extract the essence of their efficacy. The nano-transmission technology can 100% penetrate epidermis, directly reach basal cells and target the stem cells in the deep layer of your skin.

     

     

     

     

     

     

     

     

     

     

                Salvia miltiorrhiza                            Buddleja officinalis

     

    Schematic diagram of epidermal stem cell differentiation

    The skin is a layered tissue, from bottom to top, composed of subcutaneous tissues, dermis, and epidermis that contains original skin flora. The skin is the most vulnerable to external invasion, but it can constantly renew itself thanks to the stem cells in the basal layer of epidermis. These stem cells undergo a specific division process, produce new cells and further differentiate into keratogenesis cells. Under normal conditions, keratogenesis cells will automatically convert into keratinocytes and secrete intercellular gums composed of cholesterol, fatty acids and ceramides, which help to form a skin barrier function to prevent dehydration.

    Skin balance is constantly challenged by internal aging processes, UV rays, contaminants, screen light of electronic products and external stimuli, resulting in dramatic changes in the epidermal structure. Over time, due to the decreased activity of epidermal stem cells and efficiency of cell differentiation, epidermal cell renewal will slow down, and eventually, the following situations will happen to the skin:

  • The barrier function is weakened and the hydration is decreased (the barrier function of keratinocyte skin is reduced);
  • Fine lines and wrinkles appear (the number of cells and the cohesion of the

  • skin are reduced);
  • Exfoliated epidermis accumulates (reduced exfoliation process);
  • Microbial flora loses balance (unbalanced or fragile stratum corneum microecology).
  • Over time, the entire renewal process of the skin epidermis slows down due to the lower activity of epidermal stem cells and the less efficient cell differentiation process. The skin becomes dry, rough and vulnerable, and fine lines as well as wrinkles appear. Maintaining the activity of epidermal stem cells is a key factor in ensuring skin vitality and regenerative capacity. NanoStemCellTMis a new generation of UV protective active ingredient developed by PuriPharm, the main raw materials of which are Salvia miltiorrhiza and Buddleja officinalis extracts. NanoStemCellTMprovides five protections when protecting epidermal stem cells from aging caused by UV radiation:

    1) Absorption of UV rays, high-energy visible light, and blue lasers: Compared with chemical absorbents, NanoStemCellTMcan better absorb UV and blue lasers.

    2) Effective reduction in the screen light damage of electronic products: Under normal circumstances, the screen light of electronic products decreases the mitochondrial ATP synthesis rate, the type I procollagen content, and the cell proliferation activity, and lead to the change of fibroblast morphology. NamoStemCellTM effectively reduces the extent to which screen light destroys mitochondrial function and damage to fibroblast morphological structure, and reduces the adverse effects of visible light emitted by electronic screens on the skin.

    3) Restriction on the conversion of trans-UCA (urinary acid) to cis-UCA caused by UV exposure: urinary acid exists in the stratum corneum, and when exposed to UV irradiation, trans-uric acid is converted into cis-uric acid. This is the innovation of the new active ingredient of NamoStemCellTM: the active ingredient prevents the conversion of trans-urinary acid to cis-uric acid, thus limiting the adverse effect of apoptosis, immunosuppression and ROS (reactive oxygen species) formation.

    4) Reduction in lipid peroxidation: Lipid peroxidation is the process during which free radicals “steal” electrons from lipids in the cell membrane, ultimately leading to cell damage. In vitro, an additional 0.3% Nasta TMcan reduce the UV light-induced lipid peroxidation by 54%.

    5) Protection for the epidermal stem cells that maintain and restore the epidermis: in the epidermis, epidermal stem cells exist and have quite unique functions: renewing the epidermis and repairing damaged tissues by continuously forming new keratinocytes,. As the skin ages, this process will slow down while the number and activity of stem cells will decrease. Unhealthy lifestyles, UV and blue laser exposure will make this pay and cause the skin to be thiner, slacker, more wrinkled and less moisturized. Therefore, it is very important to protect these stem cells while maintaining and repairing the epidermis. NanoStemCellTMprotects and maintains the activity of epidermal stem cells and limits oxidative stress caused by exposures to UV rays and blue lasers. The new active element mainly composed of Salvia miltiorrhiza and Buddleja officinalis can prevent aging and photo-aging effects through the four mechanisms above, and protect the dermal components from the oxidative stress induced by UV rays and blue lasers.

    NanoStemCellTMplays a role in the activity of photo-protective agents for skin cells

    Skin conditions before and after the use of NanoStemCellTMactives

    In vitro, in vivo and clinical studies have shown that NanoStemCellTMhas a natural and unique skin reactivation effect. The skin activation takes effect from within through the following six synergies:

    1. Protect and activate skin stem cells
    2. Reactivate cell metabolism
    3. Stimulate cell differentiation
    4. Enhance skin barrier structure
    5. Reactivate natural exfoliation
    6. Actively protect skin microbial flora

    This versatile active ingredient will benefit users in just 2 weeks. Compared with the control one, the product has better skin moisturization and skin surface improvement with a wrinkle reduction of up to 30%.

    Biological activity: activation and protection of stem cells in vitro

    1. Cloning Ability of Stem Cells

    We cultivated human stem cells (external root sheath) for 48 hours with and without 0.5% actives, respectively, and evaluated their cloning ability with optical microscopy.

     

    Untreated stem cells                               Stem cells + 0.5%NanoStemCellTM

    Isolated stem cell                                                    Clones of stem cells

    RESULTS: Compared with the unprocessed cells, we found that the addition of actives had a significant effect on the cloning ability of stem cells, indicating that NanoStemCellTMcan maintain the cloning ability of skin stem cells.

    2.Protect stem cells from UV rays

    Stem cells + UV rays + 0.5%NanoStemCellTM

    We processed samples of human stem cells (external root sheath) with and without actives, exposed them to two doses of UVA (8J/cm2) and UVB (150mJ/cm2) respectively, and observed the expression of activating protein that survives through immunofluorescence.

    Untreated stem cell           Stem cells + UV rays

    RESULTS: NanoStemCellTMincreased the expression of activating protein that survives after UV irradiation by 171% compared with normal values, thereby protecting stem cells from apoptosis under this condition.

    3.Directly inhibit pro-apoptotic enzymes
    We added 0.1%, 0.5%, and 1%, three gradient concentrations of actives, and measured the activity of apoptosis proteases, caspase-9 and caspase-3 through spectrophotometry.

    RESULTS: The inhibition rate of NanoStemCellTMto both enzymes is 31%, indicating that the actives have anti-apoptotic effects.

    Stimulate epidermal metabolism (in vitro)

    We conducted skin explants on 8 healthy individuals (31 to 67 years old) with three processing methods — no treatment, comparison or 0.5 NanoStemCell™, and measured the expression of Ki67 tumor markers 2 days later through immunohistochemistry .

     

    RESULTS: Epidermal metabolism increased by up to +84% after NanoStemCellTMtreatment, compared with the untreated one. In addition, stem cell metabolism at the dermal-epidermal junction (DEJ) was also observed to be activated.

     

    Stimulate keratinocyte differentiation and barrier function (in vitro)

    We processed normal human keratinocytes with 0.1% NanoStemCellTMand quantified the expression of mRNA 24 hours later through q-RT-PCR.

    # T test, p = 0.06; * T test, P < 0.05;

    ** T test, P < 0.01; *** T test, P < 0.001

    RESULTS: NanoStemCellTMsignificantly increased the expression of all genes involved in keratinocyte differentiation, including those related to maintaining barrier function.

     

    Improvement of epidermal differentiation and barrier function (in vitro)

    We exfoliated reconstructed human epidermis (RHE) after 11 days to simulate the barrier defects of skin. Two days later, we adopted immunostaining to observe three proteins affected by NanoStemCellTM: involucrin, filaggrin, and loricin.

    * T test, P < 0.05;** T test, P < 0.01; *** T test, P < 0.001

    RESULTS: Compared with the control one, NanoStemCellTMresulted in significant overexpression of three protein markers (statistically), with a filaggrin expression of up to 250%, an involucrin expression of up to 490%, and a loricrin expression of up to 470%. It is obvious that NanoStemCellTMplays an extremely important role in the maintaining the epidermal differentiation and barrier function.

    Stimulate the production of skin barrier tissue (in vitro)

    The reconstructed human epidermis (RHE) was prepared from keratinocytes of a 24-year-old female donor and exposed to air for 10 days. The RHE was treated with a mixture of cytokines from D6 to D10 period to simulate barrier defects caused by local inflammatory stress. It is processed with and without 0.5% NanoStemCell™, and we analyzed the total content of ceramide by LC/MS. Such experiment was repeated for 3 times.

    * T test, P<0.05 **T test, P<0.05

    RESULTS: NanoStemCellTMstimulated an increase in the total amount of epidermal ceramide, and the ceramide content increased by 37% compared with the control one, demonstrating the ability of the skin barrier function to recover. Meanwhile, it also increased the average chain length of fatty acids attached to the main part of ceramide, raising the efficiency of barrier function . As a major ceramide, cytokines had a significant effect on the chain length ratio (C24/C18) of α-hydroxysphingosine. Adding NanoStemCellTMbrought the ratio back to the basic level, or even higher.

    Natural bio-reactivation to exfoliate (in vitro)

    On the first and the second day, we processed the skin explants from 8 healthy individuals (31 to 67 years old) in three ways — unprocessed, blank comparison and 0.5% NanoStemCell™.

  • Expression of KLK5 protease
  • 2 days later, we measured the expression of KLK5 protease through immunohistochemistry. KLK5, i.e., kallikrein-5, was involved in the exfoliation process.

    RESULTS: NanoStemCellTMsignificantly activated the expression of KLK5 compared to the unprocessed skin, resulting in an increase in KLK5 positive cells by up to + 13%. Compared to the control one, NanoStemCellTMtreatment showed the inhibition to KLK5 expression.

  • Exfoliation
  • Two days after the treatment, the experts used histological analysis to evaluate the natural exfoliation of the three explants of each donor.

    RESULTS: The epidermal exfoliation after NanoStemCellTMtreatment  visibly increased by 60% (vs. untreated skin).

     

    Efficacy assessment

    Barrier function and hydration improvement (Clinical Study 1 and Clinical Study 2)

    To evaluate the benefits of NanoStemCellTMin body care applications, we conducted the first clinical study on 19 women with average dry skin age of 39.5 years old (skin moisture value below 40 AU) through double-blind comparison. 0.5% NanoStemCellTMwere applied to their forearms twice a day; or we did a control experiment (the same formulation without actives).

    1. Skin barrier strengthening and hydration effect (Raman spectroscopy)

    After 14 days and 28 days, we measured the contents of lipid, protein and moisture of the two groups of forearm skins by Raman spectroscopy.

     

    RESULTS: NanoStemCell™ resulted in a significant increase in lipid, protein and water content of the barrier functional tissue in only 2 weeks compared to the control one. After 14 days and 28 days, they increased separately:

  • Lipid content increased by 88%
  • Protein content increased by 57%
  • Hydration increased by 38%
  • NanoStemCellTMsignificantly restored the barrier function and provided the skin with better hydration.

    2.Skin epidermal reconstruction (OCT)

    The quality (density and structure) of the volunteers’ skin tissue was analyzed at the 1st, 14th days, and 28th day. We performed the analysis by optical coherence tomography (OCT), chose to observe the contrast between epidermis and dermis, and discovered younger skin.

    RESULTS: NanoStemCellTMled to an increase in the density of the stratum corneum and a better contrast of visible DEJs (dermal epidermal junction) between epidermis and dermis. All these results were related to better and younger skin status.

    3.Skin surface renewal: exfoliation

    Using a skin diagnostic tool to study exfoliation, we conducted the second clinical double-blind control trial for care applications to measure natural skin exfoliation. The study was conducted on 20 women (average age: 44) with a dry leg skin (measured value below 35 AU), using the product or control one twice a day for 28 days.

    RESULTS: NanoStemCellTMcaused an obvious increase in skin exfoliation and surface renewal (upper epidermis) by up to 245% compared to the control group.

     

    Maintain microbial flora (Clinical Trial 3 – Skin Microbiota)

    To evaluate the benefits of NanoStemCellTMin body care applications, we selected 19 female volunteers with dry skin for the third clinical study. They were between the ages of 19 and 50, with an average age of 39.5. We applied NanoStemCellTMproducts and the control one on their left and right forearms twice a day. On the 1st and 14th days, we conducted metagenome studies such as collecting, extracting and sequencing the microbial DNA of all volunteers. A total of 24 billion DNA base pairs were analyzed by bioinformatics, and the time-varying compositions of flora under the two conditions (the relative abundance of more than 0.1% and that of more than 1%) were compared.

    Results: NanoStemCellTMhad an immediate protective effect on the skin microflora. We did not observe any significant change in the microbia on the 14th day, while in the epidermis applied with the control group, the firmicute was found to be significantly affected. In addition, compared with the control one, NanoStemCellTMactivity could inhibit the evolution of a random pathogen (Finegoldia genus) by up to -58%.

     

    NanoStemCellTMbalances and improves skin composition

    Skin Rejuvenation and Anti-Aging (Clinical Trial 4)

    The fourth 28-day clinical double-blind control trial was conducted on 15 women who had crow’s feet (aged 35 to 55; the average is 43). The volunteers applied a control product to one side of their faces, and applied 0.5% NanoStemCellTMon the other side. On the 14th and 28th days, the relaxation of their skin was analyzed through a silica gel copy and a skin image analyzer.

    RESULTS: The skin applied with NanoStemCellTMshowed a significant anti-aging effect after 14 days, the wrinkles decreased by up to 30%, and the total area of ​​wrinkles decreased by 24%.

     

    Solubility: water soluble

    Dosage: 0.1-5%

    Processing: It may be added at the end of the formulation process with gentle agitation or added directly to the aqueous phase. The preparation temperature shall be controlled below 80 ° C and the pH is between 4-8.

    Efficacy: skin renewal and activation, skin repair, skin regeneration, dry skin treatment, anti-aging, anti-wrinkle, skin moisturizing and hydration, enhanced skin barrier, natural skin exfoliation, and protection for skin microbial flora.

    Application: cream for dry skin, anti-aging night cream or day cream for face and body, body lotion for dry and thick skin, protective cream for skin microbia, organism regeneration spray, active moisturizing lotion, skin beauty products, and skin rejuvenating essence.

    NanoActive Q10(NanoActive Q10)

    Nanoactive Q10 is a novel white coenzyme Q10 nano-liposome preparation (NanoLiposomal Q10).
    Product Name: Nano Liposomal CoQ10; Nanoactive CoQ10; BioaActive CoQ10; BioActive Coenzyme Q10; NanoActive Coenzyme Q10; Water-Soluble Coenzyme Q10; Fat-Soluble Coenzyme Q10 CAS: 303-98-0

    Description: Coenzyme Q10 widely exists in mitochondria of the whole body. It has stronger antioxidant function than vitamin C and vitamin E. It can effectively eliminate free radicals and has good anti-aging, anti-wrinkle and whitening functions.” CoQ10 is a fat-soluble compound and ordinary emulsion that is difficult to be absorbed by the skin. Coenzyme Q10 Nanoactives with a particle size of less than 30 nm developed by NDS completely solves the solubility problem of CoQ10, allowing CoQ10 to be stably encapsulated in nanocarriers, which can greatly increase absorption efficiency. and bioavailability.”

    Features :

  • NanoActive Q10 uses FDA GRAS grade materials for high safety
  • NanoActive Q10 has good heat, pH and sheer stablity and is easy to use
  • NanoActive Q10 greatly improves the stability and bioavailability of CoQ10
  • NanoActive Q10 can improve the stability of other active matters in the product and facilitate absorption
  • NanoActive Q10 is 100% water soluble and 100% oil soluble (amphiphilic), allowing a wide range of uses.
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    NanoActive Q10 Diluent vs. On-market Product Diluent

     

    Efficacy:

  • NanoActive Q10 can increase the antioxidant effect of skin cells and reduce the damage from free radicals to the skin;
  • NanoActive Q10 is an active ingredient that works well to repair wrinkles, whiten and moisture skins, anti-aging, and increase skin elasticity, etc.
  • Use: Add it at ordinary temperature. Heat it to over 40℃ to speed up the dissolution. Recommended dosage: 1-5%. Better effect with products containing lipoic acid.

    Storage: Keep it away from light at a temperature below 25℃.

    CoQ10’s serial effect on the skin

     

    CoQ10 promotes secretion of collagen I from fibroblast

     

                       Before use,                                                         after use

    CoQ10’s improvement on skin texture

    NanoActive RES

    Nanoactive Res is a novel resveratrol nanoliposome preparation (NanoLiposomal Resveratrol).
    Product Name: Nano Liposomal Res, Nanoactive Res; BioActive Resveratrol; NanoActive Resveratrol CAS: 501-36-0

    Description: Resveratrol (“Res”) is a non-flavonoid polyphenolic compound. A Japanese called Tokaoka was the first one who obtained it by separating it from the roots of Veratrum granginorumin 1940. With the in-depth study of resveratrol, it has been shown that resveratrol has a wide range of activities such as anti-cancer, anti-oxidation, anti-bacteria and anti-inflammation which can be broadly used in medicine, health care products, food, cosmetics and other fields. Resveratrol has many targets in the skin, so it can be used as a skincare product to produce whitening, anti-wrinkle, anti-bacteria, anti-inflammation, acne curing and anti-aging effects. It is an emerging natural active substance with high efficiency. However, resveratrol is hardly soluble in water while easy to be oxidized and discolored, which limits its application in cosmetics. The nano actives, developed with the nano-transmission system (NDS), has a particle size of less than 30nm, and enables the resveratrol to be steadily encapsulated in nano-carriers, greatly improving the absorption efficiency and bioavailability. This overcomes the original defects while maintaining the advantages with a more convenient and wider range of uses.

     

     

     

     

     

     

       Resveratrol

                       

     

     

     

     

     

     

     

      Number of  papers on resveratrol in PUBMED over years

     

     

     

     

     

     

     

    Resveratrol can effectively prolong life span

    Skincare effect

    Resveratrol is a natural antioxidant present in plants. Its antioxidant capacity is 20-50 times that of L-C. If applied simultaneously with L-C, the effect will be multiplied. Resveratrol inhibits collagenase, reduces collagen degradation, promotes collagen proliferation, and firms skin. Resveratrol allows cells to enhance their self-defense enzymes, thereby repairing damaged DNA, while effectively inhibiting oncogenic proteins produced by UVB exposure. Resveratrol can effectively inhibit melanin synthesis by reacting with multiple targets required for melanin synthesis, thereby achieving whitening and brightening effect.

     

     

     

     

    Resveratrol inhibits melanin synthesis through multiple targets

     

    Features

  • NanoActive RES uses FDA GRAS grade materials for high safety
  • NanoActive RES is stable to heat, pH and shearing, so it is convenient to use.
  • NanoActive RES greatly improves the stability and bioavailability of resveratrol
  • NanoActive RES can improve the stability of other active matters in the product and facilitate absorption
  • NanoActive RES is 100% water soluble and 100% oil soluble (amphiphilic), allowing a wide range of uses.
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    Applications

  • Promote collagen synthesis, improve skin thickness and elasticity, remove wrinkles, and firm your skin
  • Inhibit melanin synthesis, reduce pigmentation and whiten your skin
  • Eliminate free radicals and confront skin aging
  • Promote wound healing and reduce scars
  • Cure acne and reduce acne marks.
  • Anti-bacteria, diminish inflammation and sensitization, and provide analgesic effects
  • Prevent scarring and fibrosis
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    Use:Add it at room temperature. You may heat it to over 40℃ to speed up the dissolution. Recommended dosage: 1-5%. The optimum pH range of the system is 3.0-6.5, and the finished product should be protected from direct exposure to sunlight (using opaque packaging). It is recommended to add UV absorber to day care products.

    Storage: Keep it away from light at a temperature below 25℃.

    Experimental proof

     

                         Resveratrol inhibits intracellular melanin synthesis

     

                          Resveratrol tyrosinase activity is superior to kojic acid

     

    Low concentration of resveratrol (0.001%) can reduce B16F10 cell melanin by 50%

          Two-week application can provide noticeable whitening effect

     

                           Resveratrol has obvious anti-wrinkle effect

    Resveratrol (0.001%) is effective in treating acne

    Before RES treatment                                  After RES (0.001%) treatment